High temperature sensitivity of bumblebee castes and the colony-level costs of thermoregulation in Bombus impatiens

Abstract

Physiological thermal limits often reflect species distribution, but the role that ambient temperature (Ta) plays in limiting species within their thermal environment remains unclear. Climate change-linked declines in bumblebees, an important pollinator group, leave questions regarding which aspect of their physiology is hindered under high Ta. As a eusocial species, bumblebees utilize their ability to thermoregulate as a superorganism to maintain nest temperature (Tn) within a narrow thermal window to buffer developing larvae from developmental defects. Thermoregulatory behaviours, such as thermogenesis to warm up and fanning to cool down the nest, are energetically expensive and it is uncertain how successful large colonies are at maintaining Tn within its optimal range. Using a common bumblebee species, Bombus impatiens, our study first established the critical thermal limits (CTmax) of workers, queens, drones and larvae to determine which caste is most thermally sensitive to heat. We found that larvae had significantly lower heat tolerance than adults, highlighting the importance of colonial thermoregulation. We then measured the energy expenditure of large colonies under acute thermal stress (5–40 °C) using flow-through respirometry while simultaneously quantifying Tn. Colonies that experienced Ta at or below optimal Tn (≤30 °C) were successful at thermoregulation. At 35 °C and above, however, Tn increased despite high energetic costs to the colony. Together our results demonstrate that high Ta poses a risk to colonies that fail to buffer thermally sensitive larvae from changes in Tn.