Effects of red blood cell aggregation on microparticle wall adhesion in circular microchannels

Abstract

The wall adhesion of 1 µm microparticles in human blood was studied in circular microchannels. The level of particle wall adhesion was measured for varying levels of shear rate and varying degrees of red blood cell aggregation, which was modulated by the addition of macromolecule dextran 500. The blood preparations were injected into PDMS microfluidic devices that were modified to have circular channels, better matching the geometry of physiological microcirculation compared to square channels or Couette flow systems. The circular walls of the microchannels were embedded with biotinylated phospholipids to which marginating microspheres coated with streptavidin bound. The particle wall adhesion was evaluated by counting the particles adhering to the channel wall after flushing the channel. Blood preparations of five dextran concentrations (including baseline case of 0%) were tested for four flow velocities, to quantify the effects of aggregation for varying shear rate. It was found that the level of particle wall adhesion was positively correlated with the level of RBC aggregation, particularly at low shear rates, when aggregation was enhanced. The particle adhesion was especially enhanceat aggregation levels in the range of physiological aggregation levels of whole blood, suggesting that RBC aggregation plays an important role in the dynamic of platelets and leukocytes in vivo.