Helicase Attachment to Carbon Nanotubes for DNA Sensor

Abstract

Purpose: Current DNA detection techniques require complicated procedures, specialized training, expensive equipment, invasive samples and significant amount of sample collection and processing time. The purpose of this research was to develop a rapid, accurate, non-invasive and electronic method of DNA sensing that harnesses natural unwinding properties of DNA helicase by attaching it to Carbon Nanotubes. Methods: a. A literature review on methods of attaching proteins to carbon nanotubes was conducted b. A design of the biosensor was developed based on previously reported attachment methods for other proteins c. A part of the sensor was developed by attaching DNA helicase to carbon nanotubes d. The result was tested for preservation of helicase functionality and carbon nanotube electronic structure integrity Results: a. Helicase was successfully attached to carbon nanotubes b. Helicase was found to retain its NTP hydrolysis function, DNA binding and DNA unwinding ability upon attachment c. Carbon nanotube electronic structure and function was not compromised upon attachment Conclusions: Non-specific attachment of helicase to carbon nanotubes preserves enzyme structure and function, allowing rapid DNA unwinding at an in vitro rate comparable to DNA helicase.