Differential effects of IFN-beta-1b on alpha-beta vs. gamma-delta T cells: An investigation of activation, proliferation and activation-induced apoptosis.

Abstract

$\alpha\beta$ and $\gamma\delta$ T cells are both postulated to play a role in the pathogenesis of Multiple Sclerosis (MS). IFN-$\beta$-1b (Betaseron), a novel treatment for MS, can change the natural course of MS disease, though its exact mechanism of action is unknown. Since any benefit probably involves effects of IFN-$\beta$-1b on $\alpha\beta$ or $\gamma\delta$ T cells, I derived T cells from MS patients and healthy controls and investigated the effects of IFN-$\beta$-1b on their activation, proliferation and activation-induced apoptosis. PHA, anti-CD3 and tetanus toxoid were used as stimulants for one or both T cell types in the presence of 0-1000 IU/mL IFN-$\beta$-1b. Results indicate that IFN-$\beta$-1b differentially affects $\alpha\beta$ and $\gamma\delta$ T cells both in terms of activation and apoptosis, and that MS cells exhibit differential sensitivity to these effects compared to controls. (Abstract shortened by UMI.)