Soybean peroxidase treatment of 2,4-dichlorophenol in a soil mixture.

Abstract

Soybean peroxidase (SBP) shows potential to remediate water-soil systems, yet relatively little is understood about its reaction in soil environments. While the potential may exist for the use of SBP in soil systems, too many difficulties and unknowns face the research of SBP to soil systems. This study will focus on the use of SBP in soil slurries as a stepping block to future research with soil systems. This study investigates the use of SBP to remediate 2,4-dichlorophenol (2,4-DCP) in wastewater when soil is present in the reactor vessel. Characterization of 2,4-DCP binding to soil (peat moss and silica sand) revealed that the binding was effected by the pH and the mass of the soil. The determination of enzyme kinetic constants and enzyme reactions was not examined in this study. The application of SBP to reactors with wastewater and soil has the potential to improve or decrease removal, under different reactor conditions, versus wastewater only systems. Assumptions regarding the sorption of the 2,4-DCP to soil allowed conclusions to be made regarding the activity of the SBP. (Abstract shortened by UMI.)