Les effets de traitements aux acides gras polyinsatures omega-3 a longue chaine sur la secretion d'adiponectine de preadipocytes humains differencies

Abstract

The beneficial effect of long chain omega-3 polyunsaturated fatty acids (LCn-3PUFA) on adiponectin levels has been observed in several animal and human studies after dietary treatment. The purpose of this study was to observe the effect of LCn-3PUFA on the secretion of adiponectin in differentiated human preadipocytes and determine if this effect is under the influence of PPARgamma nuclear receptors. Human adipocytes were exposed to 125muM of DHA or EPA for 24 h. Levels of adiponectin following DHA treatment increased from 0.19+/-0.02 to 0.22+/-0.01 ng/mug, or 13.6% (P<0.05) and from 0.19+/-0.02 to 0.24+/-0.06 ng/mug or 26.2% (P=0.09) after EPA treatments. After the addition of a PPARgamma inhibitor, adiponectin levels dropped of 5.8% and 12.2% for DHA and EPA, respectively. These results confirm the positive effect of LCn-3PUFA on adiponectin levels. These effects seem to be partially mediated by PPARgamma.