Tumor suppression of the DU 145 prostate cancer cell line: Implication of chromosomes 8 and 12.

Title: Tumor suppression of the DU 145 prostate cancer cell line: Implication of chromosomes 8 and 12.
Authors: Bérubé, Nathalie Guylaine.
Date: 1996
Abstract: Tumorigenesis results from the accumulation of genetic events which give rise to inappropriate behaviour of cells. Mutations which lead to cancer progression are generally classified in two major categories, that of oncogenes and tumor suppressor genes. The latter have been more difficult to identify due to their recessive nature. The application of somatic cell fusion and microcell fusion technologies presents a means to identify genes inactivated in tumor cells. We have used these methodologies to identify specific chromosomes that, once introduced into the DU 145 prostate cancer cell line, have the ability to suppress tumorigenicity. Chromosomes 8 and 12 were shown to encode tumor suppressor genes inactivated in this prostate cancer cell line. To isolate the tumor suppressor gene encoded on chromosome 12, a smaller candidate region was identified by the use of radiation hybrid technology. Molecular methods subsequently led to the identification of chromosome 12 genes displaying increased expression in suppressed hybrids compared to tumorigenic hybrids. Novel genes and genes encoded by other chromosomes also demonstrated increased expression. We suggest that these genes are involved in the cellular pathway that leads to suppression in DU 145 prostate cancer cells upon the introduction of chromosome 12. Further analysis of the genes isolated in this study have the potential to serve as prognostic markers of prostate carcinogenesis.
URL: http://hdl.handle.net/10393/9782
CollectionThèses, 1910 - 2010 // Theses, 1910 - 2010
NN16403.PDF4.39 MBAdobe PDFOpen