Multiplicity of glutamic acid decarboxylase genes: Molecular evolution and steroidal regulation.
|Title:||Multiplicity of glutamic acid decarboxylase genes: Molecular evolution and steroidal regulation.|
|Abstract:||Gamma-aminobutyric acid (GABA) is a key neurotransmitter for reproductive control. A major site for the control of GABA synthesis is the regulation of synthesis and activity of glutamic acid decarboxylase (GAD), which exists in three isoforms in goldfish. Previous sequence data suggested that GAD65 and GAD67 originated prior to teleost divergence. We have shown that this event occurred prior to 450 million years ago, before the emergence of Chondrichthyes. Previous data showed that estradiol and testosterone modulate GAD65 and GAD67 mRNA levels in sexually regressed goldfish brain. No previous data are available on the regulation of GAD3 in any animal. We found that levels of all three GADS vary seasonally. Steroid administration of testosterone, progesterone and estradiol had seasonal, brain-region and sex specific effects. This is the first evidence for seasonal variations in GAD3 and we show a likely role for progesterone in down-regulating GAD 65 and GAD67 in goldfish brain.|
|Collection||Thèses, 1910 - 2010 // Theses, 1910 - 2010|