Comparison of gene organization in the region that surrounds the 16S rRNA gene in seven different Sulfolobales.
|Title:||Comparison of gene organization in the region that surrounds the 16S rRNA gene in seven different Sulfolobales.|
|Authors:||De Moors, Anick.|
|Abstract:||Stability in genome organization has been widely studied by comparing physical and genetic maps of different genomes and, by comparing complete sequences from different genomes. Many of these comparative studies between related species have shown some measure of conservation in gene organization. In order to determine if rearrangements in gene organization occur gradually over time, a set of related species needed to be compared in detail. My research consisted of a comparative sequence analysis of a selected region of the genome of various crenarchaeotal species belonging to the order Sulfolobales. More specifically, the gene organization of an equivalent 40-50 kbp region that surrounds the 16S rRNA gene of seven related strains or species was compared. The hypothesis of this research was that rearrangements in gene organization mirror phylogenetic history. Results from the analysis showed many blocks of conserved gene organization. A qualitative tree based on these similarities in gene order was constructed for comparison with the actual distance tree based upon the 16S rRNA sequences. My hypothesis was refuted on the basis that changes in gene organization did not mirror the phylogeny. Instead, my comparative analysis supported a punctuated model of genomic evolution. The use of gene order as a parameter to build phylogenies is discussed.|
|Collection||Thèses, 1910 - 2010 // Theses, 1910 - 2010|