Identifying and characterizing agents in soy that have a potential role in diabetes development.
|Title:||Identifying and characterizing agents in soy that have a potential role in diabetes development.|
|Abstract:||To examine which component(s) of soy proteins are diabetogenic, studies were designed to look at diabetes-related soy protein fractions. In a study of diabetes-prone and control BB (BioBreeding) rats, sera from BBc (non-diabetes-prone), BBdp (diabetes-prone), BBd-U (diabetic untreated) and BBd-T (diabetic insulin-treated) rats at different risk of developing diabetes were tested against the proteins extracted from different soy protein sources. A diabetes-related protein designated S10 was identified and reactivity against two protective bands designated S6 and S13 was associated with less risk of developing diabetes. Two separate cross sectional and prospective (blood samples collected at 45 d, 70 d and 149 d or at diagnosis) studies were carried out. Western blotting results in both studies confirmed that high S10 reactivity in the pre-diabetic period was associated with diabetes. Overall Western blot analyses showed interestingly that two soy protein-fractions, S6 and S13, were related to resistance and reactivity against these two fractions may be protective and the S10 soy protein fraction appears to be highly diabetes-related and associated with diabetes. In studies using human serum, two sets of blood samples from newly diagnosed diabetic children showed the same association for the S10 soy protein fraction. Early oral dosing with soybean meal delayed the onset of diabetes and protected some of the animals from developing the disease. Pre-absorption studies showed there was an overall reduction in reactivity to soy antigens using pre-absorbed sera. This indicated some cross reactivity between food antigens and RIN cell proteins. (Abstract shortened by UMI.)|
|Collection||Thèses, 1910 - 2010 // Theses, 1910 - 2010|