Molecular Basis of GATA-4 Expression During the Early Commitment Stage of Cardiomyogenesis

Description
Title: Molecular Basis of GATA-4 Expression During the Early Commitment Stage of Cardiomyogenesis
Authors: Yilbas, Ayse Elif
Date: 2015
Abstract: Cardiovascular diseases are among the leading causes of death in North America. Currently, there are no effective treatment options for directly repairing the damaged myocardial tissue. Therefore, cell-based therapies utilizing cardiomyocytes generated from stem cells to replace necrotic tissue will be a promising approach. However, the molecular mechanisms regulating stem cell differentiation into cardiomyocytes are not fully understood. Since GATA-4 is one of the primary regulators of cradiomyogenesis, we investigated the molecular basis of GATA-4 expression during the early stages of stem cell differentiation. Using chromatin immunoprecipitation, we have observed the direct involvement of p300 in GATA-4 gene expression. We have also examined the importance ofhHistone acetylation and acetyltransferase activity on GATA-4 expression during the early stage of cardiomyogensis using the histone deactylase inhibitor Valproic Acid and the acetyltransferase inhibitor Curcumin respectively.
URL: http://hdl.handle.net/10393/31993
http://dx.doi.org/10.20381/ruor-2730
CollectionThèses, 2011 - // Theses, 2011 -
Files