Exploring the non-death function of caspase activity during cardiac hypertrophy

Title: Exploring the non-death function of caspase activity during cardiac hypertrophy
Authors: Kalynych, Sergei
Date: 2009
Abstract: Various pathological conditions that exert stress on cardiac muscle severely compromise the supply of oxygenated blood to peripheral tissues. To maintain steady cardiac output during adverse times, the myocardium is forced to undergo tissue remodeling in a process known as cardiac hypertrophy. The loss of cardiac myocytes during the transition to heart failure has been a subject of intense investigation, and this loss is hypothesized to originate from apoptosis/programmed cell death. In particular, caspase proteases have been implicated as primary components in this process. However, caspase activity has been linked to many cellular remodeling events independent of inducing cell death. Therefore, the possibility that alterations in caspase activity precipitate the cellular alterations that give rise to pathological cardiac hypertrophy was investigated in the current study: Using cultures of primary neonatal cardiomyocytes and a combination of pharmacological and biological inhibitors it is demonstrated that: (a) Physical growth of a myocyte is not dependant on activity of the apoptotic caspases; (b) Caspase activity is not necessary for the transcriptional control of natriuretic peptide production (ANF and BNP); (c) Transcriptional activity of a general stress-responsive transcription factor Nf-kB is dependent on caspase enzymatic activity in the neonatal cardiomyocyte.
URL: http://hdl.handle.net/10393/28061
CollectionTh├Ęses, 1910 - 2010 // Theses, 1910 - 2010
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