Using Machine Learning Techniques to Understand the Biophysics of Demyelination

Abstract

Demyelination is the process where the insulating layer of axons known as myelin is damaged. This affects the propagation of action potentials along axons which can have deteriorating consequences on the motor activity of an organism. Thus it is important to understand the biophysical effects of demyelination to improve the diagnostics of its diseases. We trained a Convolutional Neural Network (CNN) on Coherent anti-Stokes Raman scattering (CARS) microscope images of mice spinal cord inflicted with the demyelinating disease Experimental Autoimmune Encephalomyelitis (EAE). Our CNN was able to classify the images reliably based on clinical scores assigned to the mice. We then synthesized our own images of the spinal cord regions using a 2D Biased Random Walk. These images are simplified versions of the original CARS images and show homogenously myelinated axons, unlike the heterogeneous nerve fibres found in real spinal cords. The images were fed into the trained CNN as an attempt to develop a clinical connection to the biophysical effects of demyelination. We found that the trained CNN was indeed able to capture structural features related to demyelination which can allow us to constrain demyelination models such that they include the simulated parameters of the synthesized images.