Lau, Andrea2013-11-072013-11-0720102010Source: Masters Abstracts International, Volume: 49-02, page: 1175.http://hdl.handle.net/10393/28532http://dx.doi.org/10.20381/ruor-12587The NuA4 complex is the only essential lysine acetyltransferase in Saccharomyces cerevisiae and is evolutionarily conserved with the human Tip60 complex, which has been implicated in a wide variety of pathologies. NuA4 plays roles in processes that range from DNA repair to gluconeogenesis. To further investigate the cellular processes impacted by and to identify new targets of NuA4, a genome-wide synthetic dosage lethal screen was performed. This analysis produced an extensive network of genetic interactions, and identified a novel link between NuA4 and septin assembly and cytokinesis. I determined that NuA4 mutants have defects in septin structure, which lead to activation of the morphogenesis checkpoint. Further, I determined that NuA4 is working directly in the septin assembly pathway, potentially by regulating the protein levels of the septin Shs1. My study has revealed a new and important role for NuA4 in regulating the cell cycle and chromosome stability.81 p.enChemistry, Biochemistry.Thesis