Alvi, Azra Johanna.2009-03-202009-03-2019901990Source: Masters Abstracts International, Volume: 31-01, page: 0261.9780315680388http://hdl.handle.net/10393/5579http://dx.doi.org/10.20381/ruor-10825Our main objective was to determine if the Lymphokine Activated Killer cells (LAK) activity is restricted to Natural Killer (NK) cells or if it is also detectable in T cell subsets, especially in CD3+ T cells that are also negative for CD4 and CD8 cell surface markers. We were able to obtain highly purified subsets of NK cells, helper/inducer (CD3+4+8$-$), suppressor/cytotoxic (CD3+4$-$8+) and double negative (CD3+4$-$8$-$) T cells. The double negative T cells are now known to be a subset of $\gamma\delta$-T cells. Highly purified subsets were obtained using antibody (sheep anti-mouse IgG) coated magnetic particles and monoclonal antibodies. The purified subsets were tested for LAK activity against a standard target cell (K562), a NK resistant/LAK sensitive target cell (HTB 38), and a NK/LAK resistant target cell. We found NK cells develop LAK activity after IL-2 stimulation and that two T cell subsets (cytotoxic/suppressor and double negative) consistently showed LAK activity (3 experiments). The helper/inducer subset showed LAK activity in 2 of 3 experiments. Interleukin 4 had an inhibitory effect on the proliferative activity of IL-2 stimulated T cells and was not used in the LAK experiments. In conclusion we have found that the LAK function is a property of an heterogeneous group of lymphocyte subsets that includes NK cells and T cell subsets. (Abstract shortened by UMI.)70 p.Biology, Cell.Thesis