Gold, Frank S.2009-04-172009-04-1719691969Source: Masters Abstracts International, Volume: 45-06, page: 3121.http://hdl.handle.net/10393/11043http://dx.doi.org/10.20381/ruor-8577It is generally accepted that there are two types of cells which produce antibody: the plasma cell and a large lymphoid cell. Also it has recently been demonstrated that a single cell can produce antibody to more than one antigen (Attardi et al. 1964 a, b, c, d, e, Trentin et al. 1966). However, little work has been done on determining whether a single cell can produce different types of immunoglobulin. Nossal (1964) did a limited study in this field and he concluded that cells can produce both 19S and 7S antibody at the same time during the animal's "switchover" from 19S to 7S antibody production. The present author has tried to develop a method of detecting immunoglobulin production in single cells using Jerne's plaque technique (Jerne et al. 1963) as a basis for isolating single antibody producing cells. The plaques were removed and placed into the central well of an Ouchterlony type gel diffusion plate, on the agar surface of an Oudin type gel diffusion tube, or in a well of a slide prepared for agar electrophoresis. After a suitable incubation period, the plaques were removed, dried, and later stained. The type of protein(s) released by the cell was then detected by precipitation in the agar gel using specially prepared antisera. The results indicate that a single cell can produce more than one type of immunoglobulin at the same time.90 p.Biology, Cell.Thesis