Gage, Jessica2013-11-072013-11-0720082008Source: Masters Abstracts International, Volume: 47-01, page: 0257.http://hdl.handle.net/10393/27586http://dx.doi.org/10.20381/ruor-12155Porphyromonas gingivalis, a key pathogen in periodontal disease, promotes atherosclerosis in apolipoprotein E null (apoe-/-) mice, a well characterized mouse model of the disease. Previous studies have provided indirect evidence that P gingivalis is capable of activating the enzyme caspase-1 in vitro, while preliminary studies conducted by our laboratory have outlined a role for caspase-1 in atherosclerosis in apoe-/- mice. The present study provides direct evidence that P gingivalis is capable of activating caspase-1, and that P gingivalis is capable of gaining access to, and localizing at, sites of lesion development following an oral challenge. Oral infection with P gingivalis exacerbated the development of atherosclerosis in the aortic root of male mice competent for caspase-1 as compared to those deficient in the enzyme. Infected animals also displayed evidence of alveolar bone loss, a hallmark of periodontal disease. Our findings reveal that oral infection with P gingivalis accelerates atherosclerotic lesion development in male apoe-/- mice through the activation of caspase-1.103 p.enBiology, Molecular.Health Sciences, Dentistry.Thesis