Khan, Sarwat Tahsin2018-07-302020-07-302018-07-30http://hdl.handle.net/10393/37940http://dx.doi.org/10.20381/ruor-22198An IL-12-expressing oncolytic virus-infected cell vaccine (MG1-IL12-ICV) can prolong survival in murine models of peritoneal carcinomatosis in an NK and CD8+ T-cell dependent manner. However, MG1-IL12-ICV enhances survival but does not provide durable cures in aggressive models of established disease suggesting the presence of immunosuppressive mechanisms. Here we show that MG1-IL12-ICV can generate specific anti-tumor T-cell responses and can delay tumor growth in prophylactic models. We further demonstrate that treatment of mice bearing tumors with MG1-IL12-ICV can recruit CD4+ and CD8+ T-cells and CD11c+ dendritic cells into the tumor microenvironment (TME) and can increase NK cell activity. Regulatory T-cells and myeloid derived suppressor cells do not appear to play a role in immunosuppression following therapy, but checkpoint molecules are upregulated. Overall, this thesis provides strong evidence for the favorable anti-tumor immune TME induced by MG1-IL12-ICV and provides avenues that can be explored to further improve outcomes with MG1-IL12-ICV.enInterleukin-12Whole Cell VaccinesInfected Cell VaccineOncolytic VirusTumor MicroenvironmentTumor ImmunologyT-cellsNK cellsImmunosuppressionThesis