Renaud, Leo-P.,Wilson, Jennifer M. M.2009-03-232009-03-2320022002Source: Dissertation Abstracts International, Volume: 64-01, Section: B, page: 0100.9780612765078http://hdl.handle.net/10393/6334http://dx.doi.org/10.20381/ruor-14793Sympathetic preganglionic neurons innervating the adrenal medulla (AD-SPN) regulate the release of adrenal catecholamines into the bloodstream. This research was undertaken to investigate the intrinsic properties and synaptic pathways characteristic of AD-SPN in neonatal rat spinal cord slice preparation. The presence of Lucifer Yellow from the patch pipette and Rhodamine-Dextran-Lysine from the adrenal medulla in the same neuron post recording identified AD-SPN. Active intrinsic properties revealed and characterised include: a potassium-mediated transient outward rectification present in 96% of AD-SPN and separable into a short 4-aminopyridine- and a long barium-sensitive component; a potassium-mediated sustained outward rectification revealed in TTx, activated positive to -50mV and blocked with quinine. These conductances contribute to the repolarising phase of the action potential. 89% of AD-SPN possessed potassium-mediated anomalous inward rectification. All AD-SPN displayed a high voltage-activated calcium spike that prolongs the action potential. The addition of internal caesium (140mM) revealed a low threshold spike mediated by T-type calcium channels that serve to facilitate burst firing. 75% of AD-SPN exhibited evidence of electrotonic coupling, indicated by characteristic oscillations in membrane potential and confirmed with dual recordings from electrotonically coupled AD-SPN. Electrotonic coupling promoted synchronous activity. An enhanced afterhyperpolarising potential facilitated transient termination of action potential firing forming bursts of activity. A role for calcium in the regulation of neuronal activity via action on electrotonic coupling was suggested by caffeine (10mM) decreasing, BAPTA-AM (15muM) and calcium free aCSF increasing the junctional conductance. Electrical stimulation of the descending fibres in both the ipsi- and contralateral funiculi evoked fast EPSPs in all AD-SPN that were mediated by both NMDA and non-NMDA receptors. A subpopulation of AD-SPN received fast IPSPs mediated by GABA acting via GABAA receptors. A train of stimuli (4 x 10Hz) in ipsi- and contralateral funiculi also evoked a slow IPSP mediated by noradrenaline acting via alpha 2-adrenergic receptors to increase a potassium conductance. The results provide insight into central mechanisms that contribute to the regulation of adrenomedullary catecholamine secretion.249 p.Biology, Neuroscience.Thesis