The adaptive response: I. Assessing the relative biological effectiveness of low doses of tritium. II. Determining its effect on DNA repair at a chromosomal level.

FieldValue
dc.contributor.advisorBrown, D.,
dc.contributor.authorBroome, Ernest Jason.
dc.date.accessioned2009-03-23T17:37:13Z
dc.date.available2009-03-23T17:37:13Z
dc.date.created1999
dc.date.issued1999
dc.identifier.citationSource: Masters Abstracts International, Volume: 38-03, page: 0659.
dc.identifier.isbn9780612452060
dc.identifier.urihttp://hdl.handle.net/10393/8788
dc.identifier.urihttp://dx.doi.org/10.20381/ruor-7482
dc.description.abstractThe adaptive response, a phenomenon sensitive to low doses of ionizing radiation, was used to assess the relative biological effectiveness (RBE) of low doses of tritium. Normal human fibroblasts (AG1522) were grown in culture to confluence and exposed to tritium beta-particles or 60Co gamma-ray adapting doses ranging from 0.10 cGy to 50 cGy. These cells, and other unexposed cells, were allowed to adapt during a 3 h, 37°C incubation prior to a challenge dose of 4 Gy of 60Co gamma-rays. Cells were replated in the presence of cytochalasin B and adaption was assessed by measuring micronucleus frequency in binucleated cells. Fluorescence in situ hybridization using whole chromosome paints (WCP) was then employed to probe the chromosome content of radiation induced micronuclei in radiation-adapted and non-adapted fibroblasts. (Abstract shortened by UMI.)
dc.format.extent129 p.
dc.publisherUniversity of Ottawa (Canada)
dc.subject.classificationBiology, Molecular.
dc.titleThe adaptive response: I. Assessing the relative biological effectiveness of low doses of tritium. II. Determining its effect on DNA repair at a chromosomal level.
dc.typeThesis
dc.degree.nameM.Sc.
dc.degree.levelMasters
CollectionTh├Ęses, 1910 - 2010 // Theses, 1910 - 2010

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