Mapping the genome of Sulfolobus solfataricus P2.
|Title:||Mapping the genome of Sulfolobus solfataricus P2.|
|Authors:||Young, Felix M.|
|Abstract:||The goal of this project was to construct a contig map of the 3.0 +/- 0.1 Mbp genome of the thermoacidophilic archaeon Sulfolobus solfataricus P2. This high resolution physical map would guide the DNA sequencing of the entire genome on a cosmid-by-cosmid strategy. 60 to 70% of the genome has been cloned into cosmids which have been linked into 31 contigs by the landmark strategy and chromosome walking. The lack of coverage of the entire genome by cosmid clones may be the result of the incompatibility of the A-T rich DNA of S. solfataricus P2 with the Escherichia coli host system. The identification of insertion sequence elements in this archaeal genome suggests that it may be unstable. Attempts have been made to generate an integrated contig/macrorestriction map because of the lack of genome coverage by the cosmid clones, but this effort has been unsuccessful because of unreliable hybridization results and a lack of suitable restriction enzymes. This cosmid library will be supplemented by $\lambda$ phage clones with PCR for the final few pieces for complete coverage of the genome of S. solfataricus P2 so that DNA sequencing can be completed.|
|Collection||Thèses, 1910 - 2010 // Theses, 1910 - 2010|