Serum-Free Xenogen-Free Culture Conditions Support Human Explant-Derived Cardiac Stem Cell Growth

FieldValue
dc.contributor.authorMount, Seth
dc.date.accessioned2017-01-05T20:06:38Z
dc.date.available2018-01-05T09:30:06Z
dc.date.issued2017
dc.identifier.urihttp://hdl.handle.net/10393/35678
dc.identifier.urihttp://dx.doi.org/10.20381/ruor-635
dc.description.abstractAutologous explant-derived cardiac stem cell (EDC) therapies are a promising therapy for ischemic cardiomyopathy, but straightforward clinical translation is limited by traditional culture conditions which are often supplemented with ill-defined and xenobiotic components such as fetal bovine serum. Therefore, we investigated the influence of a commercially sourced serum-free (SF) xenogen-free medium on human EDC yield, phenotype, in vitro measures of EDC performance, and post-infarct cardiac repair using an immunodeficient mouse model of acute myocardial infarction. Despite reduced production of several pro-cardiogenic cytokines, SF EDCs promoted similar vessel formation, circulating stem cell recruitment and cardiogenic differentiation as compared to standard cultures. Transplant of SF EDCs into immunodeficient mice 1 week after myocardial infarction boosted post-ischemic repair beyond that of standard EDCs by enhancing viable myocardium within the infarct. These findings demonstrate that serum-free culture methods provide a superior cardiac-derived cell product with ready clinical translatability.
dc.language.isoen
dc.publisherUniversité d'Ottawa / University of Ottawa
dc.titleSerum-Free Xenogen-Free Culture Conditions Support Human Explant-Derived Cardiac Stem Cell Growth
dc.typeThesis
dc.contributor.supervisorDavis, Darryl R.
dc.embargo.terms2018-01-05 00:00:00
thesis.degree.nameMSc
thesis.degree.levelMasters
thesis.degree.disciplineMédecine / Medicine
uottawa.departmentMédecine Cellulaire et moléculaire / Cellular and Molecular Medicine
CollectionThèses, 2011 - // Theses, 2011 -

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