Towards the Identification of Candidate Gene(s) for Fusarium Head Blight Resistance on the 7EL Chromosome of Thinopyrum elongatum: Design and Use of Genetic Markers

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dc.contributor.authorTekieh, Farideh
dc.date.accessioned2017-01-05T17:44:03Z
dc.date.available2017-01-05T17:44:03Z
dc.date.issued2017
dc.identifier.urihttp://hdl.handle.net/10393/35670
dc.identifier.urihttp://dx.doi.org/10.20381/ruor-627
dc.description.abstractTriticum aestivum (bread wheat), one of the most globally important cereal crops, is vulnerable to fusarium head blight (FHB). The disease is mainly associated with the pathogen Fusarium graminearum and generates yield losses and mycotoxin contaminated grains with low quality. One possible solution to overcome this problem is the production of FHB resistant wheat varieties by crossing with strongly resistant germplasm from either wheat or closely related species. Thinopyrum elongatum is a wild grass that carries genetic resistance to FHB on the long arm of its chromosome 7E (7EL). In the first part of this research project, five Th. elongatum accessions were characterized for their response to F. graminearum infection. In the second part, BC1F4 progeny derived from the cross CS-ph1b × CS-7E(7D) were characterized to better define the 7E fragments introgressed into the 7D chromosome. Progeny were screened with a series of known 7E-specific genetic markers and for their FHB resistance. Among the 43 wheat plants tested, twelve FHB resistant progeny were shown to carry a similar, smaller 7EL introgressed fragment based on genetic marker screening. To characterize further the introgressed 7EL fragments, additional 7EL-specific markers as well as 7DL-specific markers for homoeologous wheat sequences were designed. As neither wheat nor Th. elongatum genomes were fully sequenced at the time, this made the designing procedure challenging; a cross-walking strategy between wheat and Th. elongatum draft genomic sequences was used. Twelve pairs of markers for homoeologous sequence regions of 7EL and 7DL chromosomes plus six individual 7EL- and four 7DL-specific markers were successfully designed. Nine novel 7EL-specific markers were associated with the smallest 7EL fragment carrying FHB resistance. That smallest introgressed 7EL fragment replaced approximately half of the 7DL chromosome, based on the absence of 7DL markers in some progeny. The novel 7EL- and 7DL-specific markers as well as the proposed genetic order for novel and previously designed markers contributed greatly to the characterization of the introgressed 7EL fragments in the 7DL chromosome. Further analysis of progeny from the next generations of these plants and from other families will be required to confirm the results and possibly obtain much smaller 7EL fragments.
dc.language.isoen
dc.publisherUniversité d'Ottawa / University of Ottawa
dc.subjectFusarium head blight
dc.subjectThinopyrum elongatum
dc.subjectTriticum aestivum
dc.subjectBread wheat
dc.subject7EL chromosome
dc.subjectFusarium graminearum
dc.subject7EL-specific markers
dc.subject7DL-specific markers
dc.subjectFHB resistance
dc.subjectGenetic order
dc.subjectwheat/7EL cross-walking strategy
dc.subjectGenetic markers
dc.titleTowards the Identification of Candidate Gene(s) for Fusarium Head Blight Resistance on the 7EL Chromosome of Thinopyrum elongatum: Design and Use of Genetic Markers
dc.typeThesis
dc.contributor.supervisorOuellet, Thérèse
thesis.degree.nameMSc
thesis.degree.levelMasters
thesis.degree.disciplineSciences / Science
uottawa.departmentBiologie / Biology
CollectionThèses, 2011 - // Theses, 2011 -

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