Parasitological correlates of Plasmodium ovale curtisi and Plasmodium ovale wallikeri infection

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dc.contributor.authorPhuong, Melissa S
dc.contributor.authorLau, Rachel
dc.contributor.authorRalevski, Filip
dc.contributor.authorBoggild, Andrea K
dc.date.accessioned2016-11-21T16:26:22Z
dc.date.available2016-11-21T16:26:22Z
dc.date.issued2016-11-10
dc.identifier.citationMalaria Journal. 2016 Nov 10;15(1):550
dc.identifier.urihttp://dx.doi.org/10.1186/s12936-016-1601-2
dc.identifier.urihttp://hdl.handle.net/10393/35480
dc.description.abstractAbstract Background Malaria, due to Plasmodium ovale, can be challenging to diagnose due to clinically mild disease and low parasite burden. Two genetically distinct sub-species of P. ovale exist: Plasmodium ovale curtisi (classic) and Plasmodium ovale wallikeri (variant). It is presently unknown if the sub-species causing infection affects performance of malaria diagnostic tests. The aim of this work was to understand how the genetically distinct sub-species, P. o. curtisi and P. o. wallikeri, affect malaria diagnostic tests. Methods Plasmodium ovale-positive whole blood specimens were sub-speciated by PCR and sequencing of 18S rRNA and dhfr-ts. Parasitaemia, morphology, pan-aldolase positivity, 18S copy number, and dhfr-ts sequences were compared between sub-species. Results From 2006 to 2015, 49 P. ovale isolates were identified, of which 22 were P. o. curtisi and 27 P. o. wallikeri; 80% were identified in the last five years, and 88% were acquired in West Africa. Sub-species did not differ by parasitaemia, 18S copy number, or pan-aldolase positivity. Lack of Schüffner’s stippling was over-represented among P. o. wallikeri isolates (p = 0.02). Several nucleotide polymorphisms between the sub-species were observed, but they do not occur at sites believed to relate to antifolate binding. Conclusions Plasmodium ovale is increasing among travellers to West Africa, although sub-species do not differ significantly by parasitologic features such as parasitaemia. Absence of Schüffner’s stippling may be a feature specific to P. o. wallikeri and is a novel finding.
dc.titleParasitological correlates of Plasmodium ovale curtisi and Plasmodium ovale wallikeri infection
dc.typeJournal Article
dc.date.updated2016-11-21T16:26:22Z
dc.language.rfc3066en
dc.rights.holderThe Author(s)
CollectionLibre accès - Publications // Open Access - Publications

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