Seed storage proteins of the globulin family are cleaved post-translationally in wheat embryos

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dc.contributor.authorKoziol, Adam G
dc.contributor.authorLoit, Evelin
dc.contributor.authorMcNulty, Melissa
dc.contributor.authorMacFarlane, Amanda J
dc.contributor.authorScott, Fraser W
dc.contributor.authorAltosaar, Illimar
dc.date.accessioned2015-12-18T10:56:25Z
dc.date.available2015-12-18T10:56:25Z
dc.date.issued2012-07-28
dc.identifier.citationBMC Research Notes. 2012 Jul 28;5(1):385
dc.identifier.urihttp://dx.doi.org/10.1186/1756-0500-5-385
dc.identifier.urihttp://hdl.handle.net/10393/33799
dc.description.abstractAbstract Background The 7S globulins are plant seed storage proteins that have been associated with the development of a number of human diseases, including peanut allergy. Immune reactivity to the wheat seed storage protein globulin-3 (Glo-3) has been associated with the development of the autoimmune disease type 1 diabetes in diabetes-prone rats and mice, as well as in a subset of human patients. Findings The present study characterized native wheat Glo-3 in salt-soluble wheat seed protein extracts. Glo-3-like peptides were observed primarily in the wheat embryo. Glo-3-like proteins varied significantly in their molecular masses and isoelectric points, as determined by two dimensional electrophoresis and immunoblotting with anti-Glo-3A antibodies. Five major polypeptide spots were identified by mass spectrometry and N-terminal sequencing as belonging to the Glo-3 family. Conclusions These results in combination with our previous findings have allowed for the development of a hypothetical model of the post-translational events contributing to the wheat 7S globulin profile in mature wheat kernels.
dc.titleSeed storage proteins of the globulin family are cleaved post-translationally in wheat embryos
dc.date.updated2015-12-18T10:56:25Z
dc.language.rfc3066en
dc.rights.holderKoziol et al.; licensee BioMed Central Ltd.
CollectionLibre accès - Publications // Open Access - Publications

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