Seed storage proteins of the globulin family are cleaved post-translationally in wheat embryos

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Title: Seed storage proteins of the globulin family are cleaved post-translationally in wheat embryos
Authors: Koziol, Adam G
Loit, Evelin
McNulty, Melissa
MacFarlane, Amanda J
Scott, Fraser W
Altosaar, Illimar
Date: 2012-07-28
Abstract: Abstract Background The 7S globulins are plant seed storage proteins that have been associated with the development of a number of human diseases, including peanut allergy. Immune reactivity to the wheat seed storage protein globulin-3 (Glo-3) has been associated with the development of the autoimmune disease type 1 diabetes in diabetes-prone rats and mice, as well as in a subset of human patients. Findings The present study characterized native wheat Glo-3 in salt-soluble wheat seed protein extracts. Glo-3-like peptides were observed primarily in the wheat embryo. Glo-3-like proteins varied significantly in their molecular masses and isoelectric points, as determined by two dimensional electrophoresis and immunoblotting with anti-Glo-3A antibodies. Five major polypeptide spots were identified by mass spectrometry and N-terminal sequencing as belonging to the Glo-3 family. Conclusions These results in combination with our previous findings have allowed for the development of a hypothetical model of the post-translational events contributing to the wheat 7S globulin profile in mature wheat kernels.
URL: http://dx.doi.org/10.1186/1756-0500-5-385
http://hdl.handle.net/10393/33799
CollectionLibre accès - Publications // Open Access - Publications
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