No training required: experimental tests support homology-based DNA assembly as a best practice in synthetic biology

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Title: No training required: experimental tests support homology-based DNA assembly as a best practice in synthetic biology
Authors: Azizi, Afnan
Lam, Wilson
Phenix, Hilary
Tepliakova, Lioudmila
Roney, Ian J
Jedrysiak, Daniel
Power, Alex
Gupta, Vaibhav
Elnour, Nada
Hanzel, Martin
Tzahristos, Alexandra C
Sarwar, Shihab
Kærn, Mads
Date: 2015-06-12
Abstract: Abstract The Registry of Standard Biological Parts imposes sequence constraints to enable DNA assembly using restriction enzymes. Alnahhas et al. (Journal of Biological Engineering 2014, 8:28) recently argued that these constraints should be revised because they impose an unnecessary burden on contributors that use homology-based assembly. To add to this debate, we tested four different homology-based methods, and found that students using these methods on their first attempt have a high probability of success. Because of their ease of use and high success rates, we believe that homology-based assembly is a best practice of Synthetic Biology, and recommend that the Registry implement the changes proposed by Alnahhas et al. to better support their use.
URL: http://dx.doi.org/10.1186/s13036-015-0006-z
http://hdl.handle.net/10393/33131
CollectionLibre accès - Publications // Open Access - Publications
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