The Interaction Between Sir3 and Sir4 is Dispensable for Silent Chromatin Spreading in Budding Yeast

FieldValue
dc.contributor.authorGerson, Rosalind J.
dc.date.accessioned2015-04-15T17:55:55Z
dc.date.available2016-04-04T08:00:08Z
dc.date.created2015
dc.date.issued2015
dc.identifier.urihttp://hdl.handle.net/10393/32232
dc.identifier.urihttp://dx.doi.org/10.20381/ruor-5046
dc.description.abstractIn Saccharomyces cerevisiae, telomeric and HM silencing requires the histone deacetylase Sir2 and the chromatin binding proteins Sir3 and Sir4, which interact to form the SIR complex. Silent chromatin formation begins with a nucleation step, followed by spreading of Sir proteins along chromatin. Overexpression of Sir3 extends silent chromatin domains, however the role of Sir protein interactions within silent chromatin extensions remains unknown. Here, we generated the Sir3 mutant, Sir3-4A, which cannot interact with Sir4 but is capable of forming silent chromatin extensions when overexpressed. Within extended silent domains, Sir2 and Sir4 enrichments are similar whether Sir3 or Sir3-4A is overexpressed, suggesting that silent chromatin extensions require Sir4 but not the interaction between Sir3 and Sir4. Tethering Sir3-4A at an HMR silencer cannot nucleate silencing in the absence of Sir3, suggesting that in addition to Sir3 recruitment, the Sir3-Sir4 interaction has at least one other function during silent chromatin nucleation.
dc.language.isoen
dc.publisherUniversité d'Ottawa / University of Ottawa
dc.subjectHeterochromatin
dc.subjectSilent chromatin
dc.subjectEpigenetics
dc.subjectGene silencing
dc.subjectHistone modifications
dc.subjectSaccharomyces cerevisiae
dc.subjectSir2
dc.subjectSir3
dc.subjectSir4
dc.subjectGene regulation
dc.subjectH3K79
dc.subjectH4K16
dc.titleThe Interaction Between Sir3 and Sir4 is Dispensable for Silent Chromatin Spreading in Budding Yeast
dc.typeThesis
dc.contributor.supervisorRudner, Adam
dc.embargo.terms2016-04-04 00:00:00
thesis.degree.nameMSc
thesis.degree.levelMasters
thesis.degree.disciplineMédecine / Medicine
uottawa.departmentBiochimie, microbiologie et immunologie / Biochemistry, Microbiology, and Immunology
CollectionThèses, 2011 - // Theses, 2011 -

Files