The Influence of Vaccinium Angustifolium (Lowbush Blueberry) Leaf Extract on Trophoblast Biology

dc.contributor.authorLy, Christina
dc.description.abstractPerturbations to extravillous trophoblast (EVT) cell migration and invasion are associated with the development of placenta-mediated diseases. Dietary polyphenols have been shown to influence cell migration and invasion in models of tumorigenesis and non-cancerous, healthy cells; however, never shown in EVT cells. We hypothesize that polyphenols present in V. angustifolium leaves will promote trophoblast migration and invasion through ERK and AKT activation. Using the HTR-8/SVneo cell line as a model for EVT cells, the leaf extract increased trophoblast migration and invasion, in an ERK- and AKT-independent manner, and had no effect on cell proliferation or viability. One major polyphenol of the leaf extract was identified and may be an active compound. We have demonstrated for the first time that V. angustifolium leaf extract increases EVT migration and invasion in vitro, thus further investigations examining potential therapeutic applications of this extract in the context of placenta-mediated diseases are warranted.
dc.publisherUniversité d'Ottawa / University of Ottawa
dc.subjectVaccinium angustifolium (lowbush blueberry)
dc.subjectExtravillous trophoblast cell biology
dc.subjectCell migration and invasion
dc.titleThe Influence of Vaccinium Angustifolium (Lowbush Blueberry) Leaf Extract on Trophoblast Biology
dc.contributor.supervisorBainbridge, Shannon
dc.embargo.terms2016-12-02 00:00:00édecine / Medicine
uottawa.departmentMédecine cellulaire et moléculaire / Cellular and Molecular Medicine
CollectionThèses, 2011 - // Theses, 2011 -