The Role of SOX7 in the Activation of Satellite Cells and Regulation of Skeletal Myogenesis

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Title: The Role of SOX7 in the Activation of Satellite Cells and Regulation of Skeletal Myogenesis
Authors: Rajgara, Rashida
Date: 2014
Abstract: One of the major drawbacks of using stem cell therapy to treat muscular dystrophies is the challenge of isolating sufficient numbers of suitable precursor cells for transplantation. As such, a deeper understanding of the molecular mechanisms involved during muscle development, which would increase the proportion of embryonic stem cells that can differentiate into skeletal myocytes, is essential. In conditional SOX7-/- mice, we observed that the loss of SOX7 in satellite cells resulted in poor differentiation and fusion. In vivo, we observed fewer Pax7+ satellite cells in the mice lacking SOX7 as well as smaller muscle fibers. RT-qPCR data also revealed that Pax7, MRF and MHC3 transcript levels were down-regulated in SOX7 knockdown mice. Surprisingly, when SOX7 was over-expressed in embryonic stem cells, we found that there was a defect in making muscle precursor cells, specifically a failure to activate Pax7 expression. Taken together, these results suggest that SOX7 expression is required for the proper regulation of skeletal myogenesis.
URL: http://hdl.handle.net/10393/31746
http://dx.doi.org/10.20381/ruor-5720
CollectionThèses, 2011 - // Theses, 2011 -
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