A method for cell type marker discovery by high-throughput gene expression analysis of mixed cell populations

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Title: A method for cell type marker discovery by high-throughput gene expression analysis of mixed cell populations
Authors: Andrade-Navarro, Miguel A
Kanji, Femina
Palii, Carmen G
Brand, Marjorie
Atkins, Harold
Perez-Iratxeta, Carol
Date: 2013
Abstract: Gene transcripts specifically expressed in a particular cell type (cell-type specific gene markers) are useful for its detection and isolation from a tissue or other cell mixtures. However, finding informative marker genes can be problematic when working with a poorly characterized cell type, as markers can only be unequivocally determined once the cell type has been isolated. We propose a method that could identify marker genes of an uncharacterized cell type within a mixed cell population, provided that the proportion of the cell type of interest in the mixture can be estimated by some indirect method, such as a functional assay.
URL: http://hdl.handle.net/10393/31652
http://www.biomedcentral.com/1472-6750/13/80
DOI: 10.1186/1472-6750-13-80
CollectionLibre accès uOttawa - Publications // uOttawa Open Access - Publications
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