A Role for the Planar Cell Polarity Pathway in Neuronal Positioning Along the AP Axis of C. elegans.

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dc.contributor.authorTanner, Raymond
dc.date.accessioned2014-08-29T17:24:54Z
dc.date.available2016-08-20T08:00:08Z
dc.date.created2014
dc.date.issued2014
dc.identifier.urihttp://hdl.handle.net/10393/31521
dc.identifier.urihttp://dx.doi.org/10.20381/ruor-5738
dc.description.abstractWe sought to investigate the role of the Planar Cell Polarity (PCP) pathway in neuronal positioning along the Anterior-Posterior (AP) axis of C. elegans, and chose the worm’s DD-type motor neurons as a model. The six DD neurons (DD1-DD6) are evenly spaced in the ventral nerve cord of wild type animals. Here we showed that mutations in core PCP genes caused DD neuron spacing and positioning defects. prkl-1 double mutant combinations with vang-1 and fmi-1 showed a suppression of the more severe prkl-1 single mutant defects, which was evidence of genetic interactions between these PCP components. We also conducted a candidate screen of Frizzled, Dishevelled, Wnt, and ROCK genes, and found that dsh-1/Dishevelled, mom-2/Wnt and let-502/ROCK also played roles in DD neuronal positioning. Both vang-1 and prkl-1 were found to function within the nervous system to guide DD neuronal positioning, and prkl-1 was further identified as playing a cell autonomous role. The origins of observed DD neuron anterior positioning defects were investigated during embryogenesis, in which 1.5 fold stage prkl-1(ok3182) embryos displayed delayed intercalation of the DD neurons. This represents a novel role for the PCP pathway in mediating DD neuronal intercalation.
dc.language.isoen
dc.publisherUniversité d'Ottawa / University of Ottawa
dc.subjectC. elegans
dc.subjectPCP
dc.subjectprkl-1
dc.subjectvang-1
dc.subjectPlanar Cell Polarity
dc.subjectintercalation
dc.subjectvan gogh
dc.subjectprickle
dc.subjectneuronal
dc.subjectnon-canonical
dc.titleA Role for the Planar Cell Polarity Pathway in Neuronal Positioning Along the AP Axis of C. elegans.
dc.typeThesis
dc.contributor.supervisorColavita, Antonio
dc.embargo.terms2016-08-20 00:00:00
thesis.degree.nameMSc
thesis.degree.levelMasters
thesis.degree.disciplineMédecine / Medicine
uottawa.departmentMédecine cellulaire et moléculaire / Cellular and Molecular Medicine
CollectionThèses, 2011 - // Theses, 2011 -

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