Function of protein kinase A in Xenopus oocyte maturation

FieldValue
dc.contributor.authorWang, Jing
dc.date.accessioned2013-11-08T16:07:02Z
dc.date.available2013-11-08T16:07:02Z
dc.date.created2006
dc.date.issued2006
dc.identifier.citationSource: Dissertation Abstracts International, Volume: 67-10, Section: B, page: 5574.
dc.identifier.urihttp://hdl.handle.net/10393/29377
dc.identifier.urihttp://dx.doi.org/10.20381/ruor-12925
dc.description.abstractLike most other vertebrate oocytes, oocytes from Xenopus laevis are physiologically arrested at the first meiotic prophase. Re-initiation of meiosis, or oocyte maturation, is triggered by progesterone. Progesterone-induced oocyte maturation is thought to involve inhibition of cAMP-dependent protein kinase (protein kinase A or PKA). However, changes in PKA activity in live oocytes have never been demonstrated. I have developed a novel approach to monitor endogenous PKA activity in live oocytes, by expressing a PKA-specific substrate in oocytes followed by monitoring its PKA phosphorylation status during progesterone-induced oocyte maturation. I demonstrate that PKA is fully activated in prophase oocytes and that progesterone causes a rapid and permanent inhibition of PKA (Chapter 3). Utilizing this PKA activity indicator in live oocytes, I further demonstrate that progesterone-induced oocyte maturation requires persistent inhibition of PKA such that critical maturation-specific proteins can be synthesized and accumulated to threshold levels necessary for activation of maturation promoting factor (MPF) (chapter 4). In addition to analyzing PKA activity dynamics during oocyte maturation, I have also provided the first biochemical evidence supporting the notion that in prophase oocytes, a constitutively activated G protein coupled receptor system is responsible for elevated cAMP and the high levels of PKA activity (chapter 2).
dc.format.extent182 p.
dc.language.isoen
dc.publisherUniversity of Ottawa (Canada)
dc.subject.classificationBiology, Molecular.
dc.titleFunction of protein kinase A in Xenopus oocyte maturation
dc.typeThesis
dc.degree.levelDoctoral
CollectionTh├Ęses, 1910 - 2010 // Theses, 1910 - 2010

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