Molecular genetic characterization of jing during Drosophila embryonic central nervous system and tracheal system development

dc.contributor.authorSedaghat, Yalda
dc.identifier.citationSource: Dissertation Abstracts International, Volume: 66-12, Section: B, page: 6520.
dc.description.abstractThe aim of the thesis was to study the molecular genetic and function offing, a novel gene having important role in central nervous system (CNS) and respiratory (tracheal) system development during Drosophila embryogenesis. We established by sequencing the gene that jing has three C2H2 zinc finger motifs, and several putative transcriptional regulatory sequences. jing plays an essential role in controlling CNS midline and tracheal cell differentiation. Mutation results in the appearance of a premature stop codon in the middle of the second zinc finger decreases survival ratio in those cells. This effect appears to be a result of the functional relationship between jing and the Egfr (Epidermal growth factor receptor) pathway in the developing CNS midline and trachea. jing is also a target of Trachealess:: Tango (Trh::Tgo) heterodimers, two members of the bHLH-PAS transcription factors, which play essential role in the development of CNS and trachea. However, jing expression is under multiple control mechanisms including auto-inhibition, inhibition by the POU domain transcription factor Drifter and the ETS transcription factor Aop. On the other hand, jing is required for the balance between repression and activation that regulates Trh- and Tgo-mediated btl transcription and subsequent growth factor levels to control signaling during the primary and secondary branching processes in the respiratory system. In conclusion, jing is a downstream target of Trh::Tgo complex and its function is required for CNS midline and tracheal cell development during Drosophila embryogenesis.
dc.format.extent291 p.
dc.publisherUniversity of Ottawa (Canada)
dc.subject.classificationBiology, Molecular.
dc.subject.classificationBiology, Neuroscience.
dc.subject.classificationBiology, Animal Physiology.
dc.subject.classificationHealth Sciences, Medicine and Surgery.
dc.titleMolecular genetic characterization of jing during Drosophila embryonic central nervous system and tracheal system development
CollectionTh├Ęses, 1910 - 2010 // Theses, 1910 - 2010

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