Targeting adenoviral vectors to cells expressing EGFRvIII using a single chain antibody fused to pIX

FieldValue
dc.contributor.authorLanthier, Robert
dc.date.accessioned2013-11-07T18:14:28Z
dc.date.available2013-11-07T18:14:28Z
dc.date.created2007
dc.date.issued2007
dc.identifier.citationSource: Masters Abstracts International, Volume: 46-03, page: 1401.
dc.identifier.urihttp://hdl.handle.net/10393/27527
dc.identifier.urihttp://dx.doi.org/10.20381/ruor-12122
dc.description.abstractCurrent adenovirus retargeting strategies are unable to target the virus to specific cell types. In this study, we investigated whether fusion of a single-chain antibody, MR1, to the capsid protein IX (pIX) could target the virus to cancer cells expressing EGFRvIII. We show that addition of an endoplasmic reticulum signal peptide to pIX-MR1 significantly increased the ability of the fusion protein to bind its ligand. Use of the human CMV promoter rather than the native pIX promoter permitted a greater accumulation of the protein within the cell. Finally, addition of the HIV-1 Tat NLS caused pIX-MR1 to relocalize to the nucleus, the site of capsid assembly. Taken together, these results provide a foundation to design Ad vectors targeted to specific cells through the use of single-chain antibodies. Ultimately, the development of a tropism modified Ad vector would result in a tailored treatment for a particular acquired genetic disease.
dc.format.extent106 p.
dc.language.isoen
dc.publisherUniversity of Ottawa (Canada)
dc.subject.classificationBiology, Cell.
dc.subject.classificationBiology, Microbiology.
dc.subject.classificationBiology, Virology.
dc.titleTargeting adenoviral vectors to cells expressing EGFRvIII using a single chain antibody fused to pIX
dc.typeThesis
dc.degree.nameM.Sc.
dc.degree.levelMasters
CollectionTh├Ęses, 1910 - 2010 // Theses, 1910 - 2010

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