Role and regulation of adrenomedullin signalling during adipogenic conversion of mouse 3T3-L1 preadipocytes

FieldValue
dc.contributor.authorMilman, Pavel
dc.date.accessioned2013-11-07T18:13:11Z
dc.date.available2013-11-07T18:13:11Z
dc.date.created2006
dc.date.issued2006
dc.identifier.citationSource: Masters Abstracts International, Volume: 44-06, page: 2710.
dc.identifier.urihttp://hdl.handle.net/10393/27157
dc.identifier.urihttp://dx.doi.org/10.20381/ruor-18566
dc.description.abstractDramatic downregulation of Adrenomedullin (AM) mRNA and peptide secretion during the induction of differentiation in 3T3-L1 preadipocytes has been reported. This observation led us to hypothesize that increased levels of AM may have inhibitory effect on 3T3-L1 preadipocyte differentiation. We, therefore, examined the effect of application of exogenous AM and its specific receptor antagonist AM22-52 on adipogenic differentiation in this cell line. We also examined mRNA regulation of CRLR, which in combination with RAMP-1, and RAMP-2 or RAMP-3 proteins, forms CGRP and AM receptors respectively. Here we report that AM stimulation on day 2 of differentiation dose dependently inhibits mRNA expression of adipocyte markers PPARgamma2, leptin, and adipsin on day 7 differentiated adipocytes, and, that this effect can be, at least partially, inhibited by pre-treatment with AM22-52. We further report on the expression of CRLR mRNA and protein, and RAMP-1 and RAMP-2 mRNA regulation during 3T3-L1 differentiation. We also report striking upregulation of RAMP-3 mRNA expression during the first day of induction of differentiation.
dc.format.extent71 p.
dc.language.isoen
dc.publisherUniversity of Ottawa (Canada)
dc.subject.classificationBiology, Molecular.
dc.subject.classificationChemistry, Biochemistry.
dc.titleRole and regulation of adrenomedullin signalling during adipogenic conversion of mouse 3T3-L1 preadipocytes
dc.typeThesis
dc.degree.nameM.Sc.
dc.degree.levelMasters
CollectionTh├Ęses, 1910 - 2010 // Theses, 1910 - 2010

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