Construction and Characterization of Gene Regulatory Networks in Yeast

FieldValue
dc.contributor.authorJedrysiak, Daniel K.
dc.date.accessioned2013-04-05T13:31:39Z
dc.date.available2013-04-05T13:31:39Z
dc.date.created2013
dc.date.issued2013
dc.identifier.urihttp://hdl.handle.net/10393/24000
dc.identifier.urihttp://dx.doi.org/10.20381/ruor-2907
dc.description.abstractTwo major roadblocks in synthetic biology are the difficulties associated with the physical assembly of gene regulatory networks (GRNs) and the lack of characterized biological parts. In this work we aimed to address both of these issues. We developed a novel method for the assembly of GRNs called Brick- Mason assembly. We have shown that the method can assemble a 6 part network in a single day and provides significant advancements over traditional cloning methods. We used BrickMason to assemble GRNs that would allow us to compare natural yeast mechanisms of repression to the steric hindrance based mechanisms that are commonly used in synthetic GRNs in yeast. Our results show that the two mechansisms of repression are not equivalent. This finding opens possibilities for using a new class of repressor in a synthetic context in yeast.
dc.language.isoen
dc.publisherUniversité d'Ottawa / University of Ottawa
dc.subjectgene
dc.subjectregulatory
dc.subjectnetwork
dc.subjectsynthetic biology
dc.subjectyeast
dc.subjectDNA
dc.subjectcloning
dc.subjectBrickMason
dc.subjectGRN
dc.subjectCYC8
dc.subjectTUP1
dc.subjectSSN6
dc.titleConstruction and Characterization of Gene Regulatory Networks in Yeast
dc.typeThesis
dc.faculty.departmentMédecine cellulaire et moléculaire / Cellular and Molecular Medicine
dc.contributor.supervisorKaern, Mads
dc.embargo.termsimmediate
dc.degree.nameMSc
dc.degree.levelmasters
dc.degree.disciplineMédecine / Medicine
thesis.degree.nameMSc
thesis.degree.levelMasters
thesis.degree.disciplineMédecine / Medicine
uottawa.departmentMédecine cellulaire et moléculaire / Cellular and Molecular Medicine
CollectionThèses, 2011 - // Theses, 2011 -

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