The Effect of Aluminium Industry Effluents on Sediment Bacterial Communities

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dc.contributor.authorGill, Hardeep
dc.date.accessioned2012-10-19T12:23:50Z
dc.date.available2012-10-19T12:23:50Z
dc.date.created2012
dc.date.issued2012
dc.identifier.urihttp://hdl.handle.net/10393/23423
dc.identifier.urihttp://dx.doi.org/10.20381/ruor-6148
dc.description.abstractThe goal of this project was to develop novel bacterial biomarkers for use in an industrial context. These biomarkers would be used to determine aluminium industry activity impact on a local ecosystem. Sediment bacterial communities of the Saguenay River are subjected to industrial effluent produced by industry in Jonquière, QC. In-situ responses of these communities to effluent exposure were measured and evaluated as potential biomarker candidates for exposure to past and present effluent discharge. Bacterial community structure and composition between control and affected sites were investigated. Differences observed between the communities were used as indicators of a response to industrial activity through exposure to effluent by-products. Diversity indices were not significantly different between sites with increased effluent exposure. However, differences were observed with the inclusion of algae and cyanobacteria. UniFrac analyses indicated that a control (NNB) and an affected site (Site 2) were more similar to one another with regard to community structure than either was to a medially affected site (Site 5) (Figure 2.4). We did not observe a signature of the microbial community structure that could be predicted with effluent exposure. Microbial community function in relation to bacterial mercury resistance (HgR) was also evaluated as a specific response to the mercury component present in sediments. Novel PCR primers and amplification conditions were developed to amplify merP, merT and merA genes belonging to the mer-operon which confers HgR (Table 5.6). To our knowledge, the roles of merP and merT have not been explored as possible tools to confirm the presence of the operon. HgR gene abundance in sediment microbial communities was significantly correlated (p < 0.05) to total mercury levels (Figure 3.4) but gene expression was not measurable. We could not solely attribute the release of Hg0 from sediments in bioreactor experiments to a biogenic origin. However, there was a 1000 fold difference in measured Hg0 release between control and affected sites suggesting that processes of natural remediation may be taking place at contaminated sites (Figure 3.7). Abundance measurements of HgR related genes represent a strong response target to the mercury immobilized in sediments. Biomarkers built on this response can be used by industry to measure long term effects of industrially derived mercury on local ecosystems. The abundance of mer-operon genes in affected sites indicates the presence of a thriving bacterial community harbouring HgR potential. These communities have the capacity to naturally remediate the sites they occupy. This remediation could be further investigated. Additional studies will be required to develop biomarkers that are more responsive to contemporary industrial activity such as those based on the integrative oxidative stress response.
dc.language.isoen
dc.publisherUniversité d'Ottawa / University of Ottawa
dc.subjectbacteria
dc.subjectheavy metal resistance
dc.subjectmercury
dc.subjectmer operon
dc.subjectsediment
dc.subjectbiomarker
dc.subjectcommunity diversity
dc.subjectmercuric reductase
dc.subjectsediment metagenome
dc.subjectenvironmental microbiology
dc.subjectindustry
dc.subjectbauxite
dc.subjectalumina
dc.subjectred mud
dc.subjectSaguenay River, QC
dc.subjectPCR
dc.subjectqPCR
dc.subjectbioreactor
dc.subjectgene
dc.subjectmerT
dc.subjectmerP
dc.subjectmerA
dc.subjectrpoB
dc.subjectkatG
dc.subjectglnA
dc.subjectriver system
dc.subjectenvironmental DNA
dc.subjectaluminum industry
dc.subjectBayer Process
dc.subjectHall–Héroult Process
dc.subject16S rRNA
dc.subjectUniFrac
dc.subjectsediment wash buffer
dc.subjectcommunity richness
dc.subjectmothur
dc.subjectribosomal database project
dc.subjectDNA sequencing
dc.subjectaluminum
dc.subjectaluminium
dc.subjectRNA
dc.subjectmetatranscriptome
dc.subjectecotoxicology
dc.subjectenvironmental contaminants
dc.subjectindustrial effluent
dc.titleThe Effect of Aluminium Industry Effluents on Sediment Bacterial Communities
dc.typeThesis
dc.faculty.departmentBiologie / Biology
dc.contributor.supervisorPoulain, Alexandre
dc.embargo.termsimmediate
dc.degree.nameMSc
dc.degree.levelmasters
dc.degree.disciplineSciences / Science
thesis.degree.nameMSc
thesis.degree.levelMasters
thesis.degree.disciplineSciences / Science
uottawa.departmentBiologie / Biology
CollectionThèses, 2011 - // Theses, 2011 -

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