Gene Expression Changes in Prostate Cells upon Exposure to Environmental Anti-androgenic Pesticide Vinclozolin

FieldValue
dc.contributor.authorPrasad, Saurabh
dc.date.accessioned2012-10-01T12:53:37Z
dc.date.available2012-10-01T12:53:37Z
dc.date.created2012
dc.date.issued2012
dc.identifier.urihttp://hdl.handle.net/10393/23355
dc.identifier.urihttp://dx.doi.org/10.20381/ruor-6101
dc.description.abstractVinclozolin (VCZ), an antiandrogenic fungicide, is an endocrine disrupting chemical that is known to possess high affinity for the androgen receptor (AR) and modulate expression of critical androgen-dependant genes in the prostate. In this study, viability and expression of AR, NKX3.1 and CYP3A4 genes were measured in androgen-sensitive prostate cells LNCaP after exposure to VCZ and VCZ treated with S9 microsomes in a time and dose dependent manner. NKX3.1 is an androgen regulated gene that plays a vital role in prostate development. CYP3A4 is involved in xenobiotic metabolism. VCZ decreased the viability at high doses after 48 hours which was slightly mitigated by treatment with S9 metabolites. Expression of NKX3.1 and CYP3A4 was upregulated while an initial downregulation of AR was observed. NKX3.1 upregulation corroborates with possibility of antiandrogens to act as androgens in LNCaP. The results illustrate that VCZ can interfere with the expression of critical prostate genes.
dc.language.isoen
dc.publisherUniversité d'Ottawa / University of Ottawa
dc.subjectEndocrine Disrupting Chemicals
dc.subjectVinclozolin
dc.subjectPesticides
dc.subjectProstate Cancer
dc.subjectGene Expression
dc.titleGene Expression Changes in Prostate Cells upon Exposure to Environmental Anti-androgenic Pesticide Vinclozolin
dc.typeThesis
dc.faculty.departmentMédecine cellulaire et moléculaire / Cellular and Molecular Medicine
dc.contributor.supervisorGomes, James
dc.contributor.supervisorKrewski, Daniel
dc.embargo.termsimmediate
dc.degree.nameMSc
dc.degree.levelmasters
dc.degree.disciplineMédecine / Medicine
thesis.degree.nameMSc
thesis.degree.levelMasters
thesis.degree.disciplineMédecine / Medicine
uottawa.departmentMédecine cellulaire et moléculaire / Cellular and Molecular Medicine
CollectionThèses, 2011 - // Theses, 2011 -

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