Effects of LTD-blocking Tat-GluR2 Peptide on Contextual Fear Memory Impairments Induced by Cannabinoids

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dc.contributor.authorKamino, Daphne
dc.date.accessioned2012-08-21T09:29:04Z
dc.date.available2012-08-21T09:29:04Z
dc.date.created2012
dc.date.issued2012
dc.identifier.urihttp://hdl.handle.net/10393/23172
dc.identifier.urihttp://dx.doi.org/10.20381/ruor-5930
dc.description.abstractThe mechanisms underlying cannabinoid impairment of fear memory is not clear. This study investigated the effects of the synthetic cannabinoid HU210 and the endocannabinoid hydrolysis inhibitor JZL 195 on fear memory following contextual fear conditioning (CFC; an animal model of fear). The long-term depression (LTD)-blocking peptide Tat-GluR2 was utilized to investigate whether the expression of cannabinoid-induced LTD (CB-LTD) is required for the cannabinoid impairment of acquisition and consolidation of contextual fear memory. HU210 reduced freezing throughout the test phase of the acquisition protocol, which was not affected by pre-administration of Tat-GluR2. High and moderate doses of HU210 reduced freezing during the first and last half, respectively, of the test phase of the consolidation protocol, which was prevented by pre-treatment with Tat-GluR2. HU210 did not affect freezing during the test phase of the retrieval protocol. Thus, these results suggest that HU210 impairs acquisition and consolidation, but not retrieval of contextual fear memory, and that in vivo CB-LTD expression is required for HU210 impairment of the consolidation, but not acquisition, of contextual fear memory. We also observed that HU210 and JZL 195 do not facilitate the acquisition of contextual fear memory extinction.
dc.language.isoen
dc.publisherUniversité d'Ottawa / University of Ottawa
dc.subjectcontextual fear conditioning
dc.subjectcannabinoid
dc.subjectHU210
dc.subjectLTD
dc.subjectTat-GluR2
dc.subjectmemory
dc.subjectfear
dc.subjectanxiety
dc.subjectCFC
dc.subjectextinction
dc.titleEffects of LTD-blocking Tat-GluR2 Peptide on Contextual Fear Memory Impairments Induced by Cannabinoids
dc.typeThesis
dc.faculty.departmentMédecine cellulaire et moléculaire / Cellular and Molecular Medicine
dc.contributor.supervisorZhang, Xia
dc.embargo.termsimmediate
dc.degree.nameMSc
dc.degree.levelmasters
dc.degree.disciplineMédecine / Medicine
thesis.degree.nameMSc
thesis.degree.levelMasters
thesis.degree.disciplineMédecine / Medicine
uottawa.departmentMédecine cellulaire et moléculaire / Cellular and Molecular Medicine
CollectionThèses, 2011 - // Theses, 2011 -

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