Circulating Progenitor Cell Therapeutic Potential Impaired by Endothelial Dysfunction and Rescued by a Collagen Matrix

FieldValue
dc.contributor.authorMarier, Jenelle
dc.date.accessioned2012-07-26T07:32:59Z
dc.date.available2012-07-26T07:32:59Z
dc.date.created2012
dc.date.issued2012
dc.identifier.urihttp://hdl.handle.net/10393/23099
dc.identifier.urihttp://dx.doi.org/10.20381/ruor-5275
dc.description.abstractAngiogenic cell therapy is currently being developed as a treatment for coronary artery disease (CAD); however, endothelial dysfunction (ED), commonly found in patients with CAD, impairs the ability for revascularization to occur. We hypothesized that culture on a collagen matrix will improve survival and function of circulating progenitor cells (CPCs) isolated from a mouse model of ED. Overall, ED decreased the expression of endothelial markers in CPCs and impaired their function, compared to normal mice. Culture of CPCs from ED mice on collagen was able to increase cell marker expression, and improve migration and adhesion potential, compared to CPCs on fibronectin. Nitric oxide production was reduced for CPCs on collagen for the ED group; however, CPCs on collagen had better viability under conditions of serum deprivation and hypoxia, compared to fibronectin. This study suggests that a collagen matrix may improve the function of therapeutic CPCs that have been exposed to ED.
dc.language.isoen
dc.publisherUniversité d'Ottawa / University of Ottawa
dc.subjectcoronary artery disease
dc.subjectcirculating progenitor cells
dc.subjectendothelial dysfunction
dc.subjectnitric oxide
dc.subjectcollagen matrix
dc.titleCirculating Progenitor Cell Therapeutic Potential Impaired by Endothelial Dysfunction and Rescued by a Collagen Matrix
dc.typeThesis
dc.faculty.departmentMédecine cellulaire et moléculaire / Cellular and Molecular Medicine
dc.contributor.supervisorSuuronen, Erik
dc.embargo.termsimmediate
dc.degree.nameMSc
dc.degree.levelmasters
dc.degree.disciplineMédecine / Medicine
thesis.degree.nameMSc
thesis.degree.levelMasters
thesis.degree.disciplineMédecine / Medicine
uottawa.departmentMédecine cellulaire et moléculaire / Cellular and Molecular Medicine
CollectionThèses, 2011 - // Theses, 2011 -

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