Role of Akt/protein kinase B in the activity of transcriptional coactivator p300

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dc.contributor.authorChen, J.
dc.contributor.authorHalappanavar, S. S.
dc.contributor.authorSt-Germain, J. R.
dc.contributor.authorTsang, B. K.
dc.contributor.authorLi, Qiao
dc.date.accessioned2012-07-24T09:08:04Z
dc.date.available2012-07-24T09:08:04Z
dc.date.created2004
dc.date.issued2004
dc.identifier.citationCellular and Molecular Life Sciences 61(13), 1675-1683.
dc.identifier.urihttp://hdl.handle.net/10393/23097
dc.description.abstractAkt/protein kinase B is a downstream target of the phosphatidylinositol 3-kinase (PI3K) pathway and plays a critical role in promotion of cell survival. The function of transcriptional coactivator p300 is required by many transcription factors to either activate or repress gene expression. Here, we show that induction of PI3K enhances the metabolic stability of endogenous p300 protein. On the other hand, repression of PI3K by LY294002 induces p300 degradation through the 26S proteasome pathway and impedes the transcriptional activity of the coactivator. In addition, Akt interacts with the coactivator and the activity of Akt is required to maintain the steady-state level of p300. Our study provides a new insight into the molecular mechanisms by which the critical concentration of p300 protein is regulated and suggests a role for Akt in control of various cellular activities through the transcriptional coactivator p300.
dc.subjectAkt
dc.subjectprotein kinase B
dc.subjectPI3K
dc.subjectp300
dc.subjectcoactivator
dc.subjectprotein degradation
dc.subjectproteasome
dc.subjectcellular signaling
dc.titleRole of Akt/protein kinase B in the activity of transcriptional coactivator p300
dc.typeArticle
dc.identifier.doi10.1007/s00018-004-4103-9
CollectionM├ędecine cellulaire et mol├ęculaire // Cellular and Molecular Medicine

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