Regulation of Adipocyte Lipolysis by TSH and its Role in Macrophage Inflammation

FieldValue
dc.contributor.authorDurand, Jason AJ
dc.date.accessioned2012-04-11T17:01:42Z
dc.date.available2012-04-11T17:01:42Z
dc.date.created2012
dc.date.issued2012
dc.identifier.urihttp://hdl.handle.net/10393/22694
dc.identifier.urihttp://dx.doi.org/10.20381/ruor-5582
dc.description.abstractElevated Thyroid-Stimulating Hormone (TSH) is associated with an increased risk of cardiovascular disease (CVD). We hypothesized that TSH-stimulated FA release from adipocytes contributes to macrophage inflammation. 3T3-L1 and human subcutaneous differentiated adipocytes were treated with TSH for 4 hours under various conditions and lipolysis assessed via glycerol secretion. Optimal conditions were determined and protein expression of ATGL, HSL and perilipin remained stable. TSH-stimulated 3T3-L1 or human adipocyte-conditioned medium (T-ACM) was placed on murine J774 or human THP-1 macrophages, respectively, and macrophage cytokine mRNA levels (IL-1β, IL-6, MCP-1, and TNFα) were measured by real-time RT-PCR. T-ACM did not change cytokine mRNA expression in J774 macrophages or THP-1 macrophages when compared to ACM. Absence of BSA in the medium may have hindered release of FA from differentiated adipocytes into the medium, BSA may be required to permit adequate FA accumulation in the medium to then evaluate the effect of T-ACM on macrophages. Further investigation is required to determine the effect of FA on J774 and THP-1 inflammatory response.
dc.language.isoen
dc.publisherUniversité d'Ottawa / University of Ottawa
dc.subjectAdipocyte
dc.subjectLipolysis
dc.subjectMacrophage
dc.subjectsubclinical hypothyroidism
dc.subjectcardiovascular disease
dc.subjectTSH
dc.subjectthyroid-stimulating hormone
dc.subjectinflammation
dc.subjectcytokine
dc.titleRegulation of Adipocyte Lipolysis by TSH and its Role in Macrophage Inflammation
dc.typeThesis
dc.faculty.departmentBiochimie, microbiologie et immunologie / Biochemistry, Microbiology and Immunology
dc.contributor.supervisorSorisky, Alexander
dc.embargo.termsimmediate
dc.degree.nameMSc
dc.degree.levelmasters
dc.degree.disciplineMédecine / Medicine
thesis.degree.nameMSc
thesis.degree.levelMasters
thesis.degree.disciplineMédecine / Medicine
uottawa.departmentBiochimie, microbiologie et immunologie / Biochemistry, Microbiology and Immunology
CollectionThèses, 2011 - // Theses, 2011 -

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