Evaluating Angiotensin II Type 1 Receptor Changes in Post- Renal Insufficiency and in Left Anterior Descending Artery Ligation Animal Models Using [11C]Methyl-Candesartan

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dc.contributor.authorMackasey, Kumiko
dc.date.accessioned2012-01-05T20:23:44Z
dc.date.available2012-01-05T20:23:44Z
dc.date.created2012
dc.date.issued2012
dc.identifier.urihttp://hdl.handle.net/10393/20504
dc.identifier.urihttp://dx.doi.org/10.20381/ruor-5118
dc.description.abstractNon invasive in vivo imaging will lead to better understanding of Angiotensin II Type 1 Receptor’s (AT1R) role in disease progression and may guide therapy in cardiovascular patients. Two models were used in this project: 5/6 nephrectomy and transient left anterior descending (LAD) ligation. Rats were scanned with [13N]ammonia and [11C]methyl-candesartan, both of which are Positron Emission Tomography (PET) tracers, at 8 weeks (nephrectomy) and 2 weeks (LAD ligation) after surgery. Western blot analysis was used to corroborate PET data. Nephrectomy: Renal AT1R image analysis displayed a 40% decrease in kidney AT1R in nephrectomized animals compared to sham (p<0.05) which was confirmed with Western blot and biodistribution. LAD ligation: Left Ventricle AT1R Western blot analysis exhibited a 60% increase in 20min ligation (p<0.05) with maintained myocardial blood flow. In conclusion, changes in renal AT1R were successfully imaged using [11C]methyl-candesartan in nephrectomized animals, and 20min LAD ligation/reperfusion is an appropriate model to image an increase in cardiac AT1R following ischemic injury.
dc.language.isoen
dc.publisherUniversité d'Ottawa / University of Ottawa
dc.subjectAngiotensin II Type 1 Receptor
dc.subject11C]Methyl-Candesartan
dc.subjectTherapy in cardiovascular patients
dc.subjectATIR
dc.subjectLeft Anterior Descending Artery Ligation /Reperfusion
dc.titleEvaluating Angiotensin II Type 1 Receptor Changes in Post- Renal Insufficiency and in Left Anterior Descending Artery Ligation Animal Models Using [11C]Methyl-Candesartan
dc.typeThesis
dc.faculty.departmentMédecine cellulaire et moléculaire / Cellular and Molecular Medicine
dc.contributor.supervisorDaSilva, Jean N
dc.contributor.supervisorBeanlands, Robert
dc.embargo.termsimmediate
dc.degree.nameMSc
dc.degree.levelmasters
dc.degree.disciplineMédecine / Medicine
thesis.degree.nameMSc
thesis.degree.levelMasters
thesis.degree.disciplineMédecine / Medicine
uottawa.departmentMédecine cellulaire et moléculaire / Cellular and Molecular Medicine
CollectionThèses, 2011 - // Theses, 2011 -

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