Mitofusin 1 and Mitofusin 2 Function in the Context of Brain Development

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dc.contributor.authorHamze, Carmen
dc.date.accessioned2011-11-01T21:09:55Z
dc.date.available2011-11-01T21:09:55Z
dc.date.created2011
dc.date.issued2011
dc.identifier.urihttp://hdl.handle.net/10393/20347
dc.identifier.urihttp://dx.doi.org/10.20381/ruor-4991
dc.description.abstractMitofusin 1 and 2 are outer-mitochondrial membrane proteins that have been shown to be involved in fusion. Mitofusin 2 has also been associated with apoptosis and development. When Mfn1 and Mfn2 were each conditionally knocked out from the cerebellum, Purkinje cells in Mfn2 deficient cerebellum during development had undergone neurodegeneration. Mutations in Mfn2 have also been associated with the Charcot Marie Tooth Type 2A (CMT2A). We want to asses the effect Mfn2 and Mfn1 might have on the development of other regions of the brain such as the telencephalon. We generated Mfn1 and Mfn2 conditional knockouts in the telencephalon by crossing them with Foxg1 Cre - a cre expressed in the telencephalon. We found that Mfn1 deficient mice have lost their corpus callosum at the midline, but survive over 6 months with a decrease in progenitor cells postnatally. Mfn2 deficient mice die between P9 and P12 with a decrease in progenitor cells postnatally and a decrease in number of neurons in the cortex. Therefore, our results suggest that Mfn1 and Mfn2 play a significant role in the development of the telencephalon.
dc.language.isoen
dc.publisherUniversité d'Ottawa / University of Ottawa
dc.subjectMfn1
dc.subjectMfn2
dc.subjectprogenitor cells
dc.subjectpost-mitotic cortical neurons
dc.subjectdevelopment
dc.subjecttelencephalon
dc.titleMitofusin 1 and Mitofusin 2 Function in the Context of Brain Development
dc.typeThesis
dc.faculty.departmentMédecine cellulaire et moléculaire / Cellular and Molecular Medicine
dc.contributor.supervisorSlack, Ruth
dc.embargo.termsimmediate
dc.degree.nameMSc
dc.degree.levelmasters
dc.degree.disciplineMédecine / Medicine
thesis.degree.nameMSc
thesis.degree.levelMasters
thesis.degree.disciplineMédecine / Medicine
uottawa.departmentMédecine cellulaire et moléculaire / Cellular and Molecular Medicine
CollectionThèses, 2011 - // Theses, 2011 -

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