The Role of XRCC1 in the Repair of DNA Strand Breaks in Skeletal Muscle Differentiation

FieldValue
dc.contributor.authorBurns, Leanne E.
dc.date.accessioned2011-09-22T14:07:01Z
dc.date.available2012-09-22T07:00:18Z
dc.date.created2011
dc.date.issued2011
dc.identifier.urihttp://hdl.handle.net/10393/20225
dc.identifier.urihttp://dx.doi.org/10.20381/ruor-6056
dc.description.abstractCaspase-3 has demonstrated a non-apoptotic function in several developmental programs including skeletal muscle differentiation, yet the mechanism of action has not been fully elucidated. Under apoptotic conditions Caspase-3 induces DNA fragmentation through activation of CAD. Recent observations have demonstrated CAD activity and the resulting DNA strand breaks are also vital for skeletal muscle differentiation. These breaks are transient in nature, suggesting an active DNA repair program to maintain genomic integrity. The aim of this study was to delineate the DNA repair mechanism coordinated with caspase/CAD mediated DNA damage. It was found that XRCC1 formed punctate nuclear foci early in myoblast differentiation concurrent to the induction of DNA damage. Caspase-3 inhibition caused attenuation of the formation of DNA lesions and XRCC1 foci in differentiating myoblasts. Targeted reduction in XRCC1 expression impaired myoblast differentiation. These results suggest that XRCC1 may play a role in repairing the DNA damage associated with myoblast differentiation.
dc.language.isoen
dc.publisherUniversité d'Ottawa / University of Ottawa
dc.subjectXRCC1
dc.subjectCaspase 3
dc.subjectCaspase activated DNase
dc.subjectdifferentiation
dc.subjectSkeletal muscle
dc.subjectDNA repair
dc.titleThe Role of XRCC1 in the Repair of DNA Strand Breaks in Skeletal Muscle Differentiation
dc.typeThesis
dc.faculty.departmentMédecine cellulaire et moléculaire / Cellular and Molecular Medicine
dc.contributor.supervisorMegeney, Lynn A.
dc.embargo.terms1 year
dc.degree.nameMSc
dc.degree.levelmasters
dc.degree.disciplineMédecine / Medicine
thesis.degree.nameMSc
thesis.degree.levelMasters
thesis.degree.disciplineMédecine / Medicine
uottawa.departmentMédecine cellulaire et moléculaire / Cellular and Molecular Medicine
CollectionThèses, 2011 - // Theses, 2011 -

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