Promoter context determines the role of proteasome in ligand-dependent occupancy of retinoic acid responsive elements

FieldValue
dc.contributor.authorLi, Qiao
dc.contributor.authorChen, Jihong
dc.contributor.authorAbed, Mahmoud
dc.contributor.authorHigazi, Aliaa
dc.date.accessioned2011-01-18T14:42:17Z
dc.date.available2011-01-18T14:42:17Z
dc.date.created2011
dc.date.issued2011-01-18
dc.identifier.urihttp://hdl.handle.net/10393/19698
dc.description.abstractRetinoid acid receptors are DNA-binding proteins mediating the biological effects of ligands through transcriptional activation. It is known that the activity of the 26S proteasome is important for nuclear receptor-activated gene transcription. However, the molecular mechanism by which the 26S proteasome participates in this process is not well understood. Here we report that the proteasome activity is essential for ligand-dependent interaction of RAR with its co-regulators such as SRC, p300 and RXR. We also determined that the proteasome activity is required for the association of liganded RAR to the genomic DNA and, consequently, for the recruitment of the coactivator complex to the retinoic acid responsive elements. Moreover, the requirement of proteasome activity for the activator activity of RAR is determined by the promoter context. Our study suggests that the 26S proteasome regulates directly the activity of RAR as an activator.
dc.language.isoen
dc.subjectgene regulation
dc.subjecttranscriptional coactivator
dc.subjectnuclear receptor
dc.subjectthe 26S proteasome
dc.subjectgenomic association
dc.titlePromoter context determines the role of proteasome in ligand-dependent occupancy of retinoic acid responsive elements
dc.typeArticle
CollectionPathologie et médecine de laboratoire // Pathology and Laboratory Medicine
Publications en libre accès financées par uOttawa // uOttawa financed open access publications

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