Serological identification and characterization of new HLA class I and non-HLA antigens.
|Title:||Serological identification and characterization of new HLA class I and non-HLA antigens.|
|Authors:||Yang, Edward Kuo-Liang.|
|Abstract:||This report embarks on an investigation of the ethnic diversity, using alloantisera, of three groups of HLA class I antigens, namely A2, A11, and C-locus antigens, and to define antigens that might be restricted to certain ethnic populations. As transplantation therapy has been widely accepted as the choice of treatment for patients with severe haematological disorders, such as acute leukemia, it is essential to match MHC antigens of unrelated donor and recipient pairs to avoid graft rejection or graft-versus-host disease and to reduce administration of immune suppressants. Results obtained from this study indicate that HLA-A2 consists of at least four variants as measured by isoelectric focusing assay. However, only three serological variants are demonstrated by alloantisera. Results further confirmed that two variants of HLA-A11 exist, as measured by serology and isoelectric focusing. The more frequent variant, A11.1, is found in donors of all ethnic backgrounds. The less frequent variant, A11.2, is found only in Oriental populations. This study also reveals two "new" serologically defined HLA-C locus antigens: HLA-Cw Hunt and HLA-Cw Grace. These two "new" antigens are found in Black, Caucasian, East Indian and Oriental ethnic groups. Identification of these two new C-locus antigens reveals that approximately 11% of the population was falsely typed and possess only one or no C-locus antigen. This study also identifies for the first time an alloantiserum that defines an ethnic-specific human minor histocompatibility antigen, hmH$\Phi,$ found only in the Oriental population with a frequency of approximately 5.5%. It was not found in 1,000 Caucasian, 200 East Indian, and 60 Black blood donors. (Abstract shortened by UMI.)|
|Collection||Thèses, 1910 - 2010 // Theses, 1910 - 2010|